Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.
Identifieur interne : 000179 ( Main/Exploration ); précédent : 000178; suivant : 000180Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.
Auteurs : Sheng-Chun Yu [Royaume-Uni] ; Florian Kuemmel [Royaume-Uni] ; Maria-Nefeli Skoufou-Papoutsaki [Royaume-Uni] ; Pietro D. Spanu [Royaume-Uni]Source :
- MicrobiologyOpen [ 2045-8827 ] ; 2019.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Complexe-1 cible mécanistique de la rapamycine (métabolisme), Génétique microbienne (méthodes), Milieux de culture (composition chimique), Protéines de Saccharomyces cerevisiae (métabolisme), Saccharomyces cerevisiae (croissance et développement), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Techniques de transfert de gènes (MeSH), Transduction du signal (MeSH), Transformation génétique (MeSH).
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Saccharomyces cerevisiae.
- génétique : Saccharomyces cerevisiae.
- métabolisme : Acides aminés, Complexe-1 cible mécanistique de la rapamycine, Protéines de Saccharomyces cerevisiae, Saccharomyces cerevisiae.
- méthodes : Génétique microbienne.
- Techniques de transfert de gènes, Transduction du signal, Transformation génétique.
English descriptors
- KwdEn :
- Amino Acids (metabolism), Culture Media (chemistry), Gene Transfer Techniques (MeSH), Genetics, Microbial (methods), Mechanistic Target of Rapamycin Complex 1 (metabolism), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (growth & development), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (metabolism), Signal Transduction (MeSH), Transformation, Genetic (MeSH).
- MESH :
- chemical , chemistry : Culture Media.
- chemical , metabolism : Amino Acids, Mechanistic Target of Rapamycin Complex 1, Saccharomyces cerevisiae Proteins.
- genetics : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Genetics, Microbial.
- Gene Transfer Techniques, Signal Transduction, Transformation, Genetic.
Abstract
Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases transformation efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitin-mediated endocytosis. We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes. Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast transformation. Based on our previous study, activation of this pathway results in up to a 200-fold increase in transformation efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.
DOI: 10.1002/mbo3.730
PubMed: 30311441
PubMed Central: PMC6528558
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Spanu</name><affiliation wicri:level="3"><nlm:affiliation>Department of Life Sciences, Imperial College London, London, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Life Sciences, Imperial College London, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author></analytic><series><title level="j">MicrobiologyOpen</title><idno type="eISSN">2045-8827</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acids (metabolism)</term><term>Culture Media (chemistry)</term><term>Gene Transfer Techniques (MeSH)</term><term>Genetics, Microbial (methods)</term><term>Mechanistic Target of Rapamycin Complex 1 (metabolism)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (metabolism)</term><term>Signal Transduction (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Complexe-1 cible mécanistique de la rapamycine (métabolisme)</term><term>Génétique microbienne (méthodes)</term><term>Milieux de culture (composition chimique)</term><term>Protéines de Saccharomyces cerevisiae (métabolisme)</term><term>Saccharomyces cerevisiae (croissance et développement)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Techniques de transfert de gènes (MeSH)</term><term>Transduction du signal (MeSH)</term><term>Transformation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Mechanistic Target of Rapamycin Complex 1</term><term>Saccharomyces cerevisiae Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetics, Microbial</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Complexe-1 cible mécanistique de la rapamycine</term><term>Protéines de Saccharomyces cerevisiae</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génétique microbienne</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Transfer Techniques</term><term>Signal Transduction</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Techniques de transfert de gènes</term><term>Transduction du signal</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases transformation efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitin-mediated endocytosis. We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes. Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast transformation. Based on our previous study, activation of this pathway results in up to a 200-fold increase in transformation efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30311441</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">2045-8827</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><Issue>5</Issue><PubDate><Year>2019</Year><Month>05</Month></PubDate></JournalIssue><Title>MicrobiologyOpen</Title><ISOAbbreviation>Microbiologyopen</ISOAbbreviation></Journal><ArticleTitle>Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signaling.</ArticleTitle><Pagination><MedlinePgn>e00730</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/mbo3.730</ELocationID><Abstract><AbstractText>Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases transformation efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitin-mediated endocytosis. We created mutants of the TORC1 pathway, alpha-arrestins, and eisosome-related genes. Our results demonstrate that the TORC1-Npr1-Art1/Rsp5 pathway regulates yeast transformation. Based on our previous study, activation of this pathway results in up to a 200-fold increase in transformation efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.</AbstractText><CopyrightInformation>© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Sheng-Chun</ForeName><Initials>SC</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, Imperial College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuemmel</LastName><ForeName>Florian</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, Imperial College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Skoufou-Papoutsaki</LastName><ForeName>Maria-Nefeli</ForeName><Initials>MN</Initials><AffiliationInfo><Affiliation>Department of Life Sciences, Imperial College London, London, UK.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Spanu</LastName><ForeName>Pietro D</ForeName><Initials>PD</Initials><Identifier 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uniqKey="Skoufou Papoutsaki M" first="Maria-Nefeli" last="Skoufou-Papoutsaki">Maria-Nefeli Skoufou-Papoutsaki</name><name sortKey="Spanu, Pietro D" sort="Spanu, Pietro D" uniqKey="Spanu P" first="Pietro D" last="Spanu">Pietro D. 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